Rapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR

O'Grady, Justin, Ruttledge, Margaret, Sedano-Balbás, Sara, Smith, Terry J., Barry, Thomas and Maher, Majella (2009) Rapid detection of Listeria monocytogenes in food using culture enrichment combined with real-time PCR. Food Microbiology, 26 (1). pp. 4-7. ISSN 0740-0020

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Abstract

A rapid method for the detection of Listeria monocytogenes in foods combining culture enrichment and real-time PCR was compared to the ISO 11290-1 standard method. The culture enrichment component of the rapid method is based on the ISO standard and includes 24h incubation in half-Fraser broth, 4h incubation in Fraser broth followed by DNA extraction and real-time PCR detection of the ssrA gene of L. monocytogenes. An internal amplification control, which is co-amplified with the same primers as the L. monocytogenes DNA, was also included in the assay. The method has a limit of detection of 1-5CFU/25g food sample and can be performed in 2 working days compared to up to 7days for the ISO standard. A variety of food samples from retail outlets and food processing plants (n=175) and controls (n=31) were tested using rapid and conventional methods. The rapid method was 99.44% specific, 96.15% sensitive and 99.03% accurate when compared to the standard method. This method has the potential to be used as an alternative to the standard method for food quality assurance providing rapid detection of L. monocytogenes in food.

Item Type:	Article
Uncontrolled Keywords:	listeria monocytogenes,real-time pcr,detection,food,tmrna,internal amplification control
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018)
Depositing User:	Justin O'Grady
Date Deposited:	10 Sep 2013 04:53
Last Modified:	10 Jan 2024 01:21
URI:	https://ueaeprints.uea.ac.uk/id/eprint/41310
DOI:	10.1016/j.fm.2008.08.009

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