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ToolsNeufeld, Josh D., Chen, Yin, Dumont, Marc G. and Murrell, J. Colin (2008) Marine methylotrophs revealed by stable-isotope probing, multiple displacement amplification and metagenomics. Environmental Microbiology, 10 (6). pp. 1526-1535. ISSN 1462-2920
Full text not available from this repository. (Request a copy)Abstract
The concentrations of one-carbon substrates that fuel methylotrophic microbial communities in the ocean are limited and the specialized guilds of bacteria that use these molecules may exist at low relative abundance. As a result, these organisms are difficult to identify and are often missed with existing cultivation and gene retrieval methods. Here, we demonstrate a novel proof of concept: using environmentally-relevant substrate concentrations in stable-isotope probing (SIP) incubations to yield sufficient DNA for large-insert metagenomic analysis through multiple displacement amplification (MDA). A marine surface-water sample was labelled sufficiently by incubation with near in situ concentrations of methanol. Picogram quantities of labelled (13)C-DNA were purified from caesium chloride gradients, amplified with MDA to produce microgram amounts of high-molecular-weight DNA (
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | sdg 14 - life below water ,/dk/atira/pure/sustainabledevelopmentgoals/life_below_water |
| Faculty \ School: | Faculty of Science > School of Environmental Sciences University of East Anglia Research Groups/Centres > Theme - ClimateUEA |
| UEA Research Groups: | Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Environmental Biology |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 27 Mar 2012 15:15 |
| Last Modified: | 20 Mar 2023 15:31 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/38517 |
| DOI: | 10.1111/j.1462-2920.2008.01568.x |
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