Eukarya 18S rRNA gene diversity in the sea surface microlayer: implications for the structure of the neustonic microbial loop

Cunliffe, Michael and Murrell, J. Colin (2010) Eukarya 18S rRNA gene diversity in the sea surface microlayer: implications for the structure of the neustonic microbial loop. The ISME Journal, 4 (3). pp. 455-458. ISSN 1751-7362

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Abstract

We have previously shown that there is a consistent and reproducible bacterioneuston community in the surface microlayer during a fjord mesocosm experiment. One possible cause of the surface microlayer-specific bacterial community is a surface microlayer-specific protist community selectively grazing on the bacterioneuston. We determined protist community structures using Eukarya 18S rRNA gene denaturing gradient gel electrophoresis (DGGE) and subsequent DGGE band sequencing using DNA samples that were collected from the surface microlayer and subsurface water of the mesocosms. As with bacterial communities, protist community structure was consistently different in the surface microlayer when compared with subsurface water. In particular, the protist community in the surface microlayer was dominated by Cercozoa, which were not detected in the subsurface water, and Ciliophora

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
University of East Anglia Research Groups/Centres > Theme - ClimateUEA
UEA Research Groups: Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation
Faculty of Science > Research Groups > Environmental Biology
Depositing User: Rhiannon Harvey
Date Deposited: 27 Mar 2012 10:40
Last Modified: 20 Mar 2023 15:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/38488
DOI: 10.1038/ismej.2009.133

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