Tools
Tools
Zhou, Liang, Singleton, Chloe, Hecht, Oliver, Moore, Geoffrey R. and Le Brun, Nick E. 
ORCID: https://orcid.org/0000-0001-9780-4061
(2012)
Cu(I)- and proton-binding properties of the first N-terminal soluble domain of Bacillus subtilis CopA.
FEBS Journal, 279 (2).
pp. 285-298.
ISSN 1742-4658
Abstract
CopA, a P‐type ATPase transporter involved in copper detoxification in Bacillus subtilis, contains two soluble Atx1‐like domains separated by a short linker at its N‐terminus, an arrangement that occurs widely in copper transporters from both prokaryotes and eukaryotes. Both domains were previously found to bind Cu(I) with very high affinity. Above a level of 1 Cu(I) per CopAab, dimerization occurred, leading to a highly luminescent multinuclear Cu(I) species [Singleton C & Le Brun NE (2009) Dalton Trans, 688–696]. To try to understand the contributions of each domain to the complex Cu(I)‐binding behaviour of this and related proteins, we purified a wild‐type form of the first domain (CopAa). In isolation, the domain bound Cu(I) with very high affinity (K = ∼ 1 × 1018 m−1) and underwent Cu(I)‐mediated protein association, resulting in a mixture of dimer and tetramer species. Addition of further Cu(I) up to 1 Cu(I) per CopAa monomer led to a weakly luminescent species, whereas further additions [2 Cu(I) per CopAa monomer] resulted in protein unfolding. Analysis of the MTCAAC binding motif Cys residue acid–base properties revealed pKa values of 5.7 and 7.3, consistent with the pH dependence of Cu(I) binding, and with the proposal that low proton affinity is associated with high Cu(I) affinity. Finally, Cu(I) exchange between CopAa and the chelator bathocuproine sulfonate revealed rapid exchange in both directions, demonstrating an interaction between the protein and the chelator that catalyses metal ion transfer. Overall, CopAa exhibits similarities to CopAab in terms of affinity and complexity of Cu(I) binding, but the details of Cu(I) binding are distinct.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Groups > Chemistry of Life Processes |
| Depositing User: | Users 2731 not found. |
| Date Deposited: | 21 Mar 2012 21:49 |
| Last Modified: | 24 Sep 2024 08:57 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/38433 |
| DOI: | 10.1111/j.1742-4658.2011.08422.x |
Actions (login required)
 |
View Item |