Diversity in natural product families is governed by more than enzyme promiscuity alone: establishing control of the pacidamycin portfolio

Grüschow, Sabine, Rackham, Emma J. and Goss, Rebecca J. M. (2011) Diversity in natural product families is governed by more than enzyme promiscuity alone: establishing control of the pacidamycin portfolio. Chemical Science, 2 (11). pp. 2182-2186. ISSN 2041-6520

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Abstract

As with many other antibiotics, pacidamycins are produced as a suite of related compounds. Unlike most other secondary metabolites, however, this diversity is not solely the result of the substrate promiscuity of the biosynthetic enzymes but also arises from a gene duplication event (Pac21, Pac21h) and control of the precursor pool (PhhA). We are demonstrating the ability to harness these three levels of control in order to direct the selective production of specific members of this family of metabolites in a “dial-a-molecule” fashion. Furthermore, PhhA is shown to be a phenylalanine 3-hydroxylase, the first of the iron- and tetrahydropterin-dependent aromatic amino acid hydroxylases to be characterised with this regioselectivity.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry
Depositing User:	Users 2731 not found.
Date Deposited:	20 Mar 2012 11:46
Last Modified:	22 Nov 2022 10:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/38358
DOI:	10.1039/c1sc00378j

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