Regulation of the divergentguaBAandxseApromoters of Escherichia coliby the cyclic AMP receptor protein

Hutchings, Matthew I. and Drabble, William T. (2000) Regulation of the divergentguaBAandxseApromoters of Escherichia coliby the cyclic AMP receptor protein. FEMS Microbiology Letters, 187 (2). pp. 115-122. ISSN 1574-6968

Full text not available from this repository. (Request a copy)

Abstract

The gua promoter (guaP) of Escherichia coli resembles those for ribosomal RNA (rrn) operons and lies in a close back-to-back arrangement with the promoter for xseA (xseP). Transcription from guaP is subject to stringent control and growth-rate-dependent regulation, and to repression by DnaA and PurR. In addition, transcription from guaP is regulated by the cyclic AMP receptor protein (CRP). Plasmid-borne promoter fusions to the receptor gene for chloramphenicol acetyl transferase were used to assess the role of CRP in controlling transcription from guaP and xseP following a downshift of cultures from rich into minimal medium. CRP is required to activate guaBA transcription and repress xseA transcription following downshift. Bandshift assays with a DNA fragment carrying the divergent promoters revealed specific binding of CRP. We propose that CRP, binding to a near-consensus site centred at -117.5, activates transcription from guaP and obstructs transcription from the xseA promoter.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Users 2731 not found.
Date Deposited: 18 Jan 2012 11:50
Last Modified: 17 Mar 2020 18:01
URI: https://ueaeprints.uea.ac.uk/id/eprint/36241
DOI: 10.1111/j.1574-6968.2000.tb09146.x

Actions (login required)

View Item View Item