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ToolsHutchings, Matthew I. and Drabble, William T. (2000) Regulation of the divergentguaBAandxseApromoters of Escherichia coliby the cyclic AMP receptor protein. FEMS Microbiology Letters, 187 (2). pp. 115-122. ISSN 1574-6968
Full text not available from this repository. (Request a copy)Abstract
The gua promoter (guaP) of Escherichia coli resembles those for ribosomal RNA (rrn) operons and lies in a close back-to-back arrangement with the promoter for xseA (xseP). Transcription from guaP is subject to stringent control and growth-rate-dependent regulation, and to repression by DnaA and PurR. In addition, transcription from guaP is regulated by the cyclic AMP receptor protein (CRP). Plasmid-borne promoter fusions to the receptor gene for chloramphenicol acetyl transferase were used to assess the role of CRP in controlling transcription from guaP and xseP following a downshift of cultures from rich into minimal medium. CRP is required to activate guaBA transcription and repress xseA transcription following downshift. Bandshift assays with a DNA fragment carrying the divergent promoters revealed specific binding of CRP. We propose that CRP, binding to a near-consensus site centred at -117.5, activates transcription from guaP and obstructs transcription from the xseA promoter.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Organisms and the Environment |
| Depositing User: | Users 2731 not found. |
| Date Deposited: | 18 Jan 2012 11:50 |
| Last Modified: | 20 Jun 2023 14:43 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/36241 |
| DOI: | 10.1111/j.1574-6968.2000.tb09146.x |
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