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Robinson, Stephen D. 
ORCID: https://orcid.org/0000-0002-6606-7588, Reynolds, Louise E., Kostourou, Vassiliki, Reynolds, Andrew R., da Silva, Rita Graça, Tavora, Bernardo, Baker, Marianne, Marshall, John F. and Hodivala-Dilke, Kairbaan M.
(2009)
avβ3 Integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis.
Journal of Biological Chemistry, 284 (49).
pp. 33966-33981.
ISSN 0021-9258
Abstract
Both vascular endothelial growth factor receptors (VEGFR) and integrins are major regulators of VEGF-induced angiogenesis. Previous work has shown that ß3 integrin can regulate negatively VEGFR2 expression. Here we show that ß3 integrin can regulate negatively VEGF-mediated angiogenesis by limiting the interaction of the co-receptor NRP1 (neuropilin-1) with VEGFR2. In the presence of avß3 integrin, NRP1 contributed minimally to VEGF-induced angiogenic processes in vivo, ex vivo, and in vitro. Conversely, when ß3 integrin expression is absent or low or its function is blocked with RGD-mimetic inhibitors, VEGF-mediated responses became NRP1-dependent. Indeed, combined inhibition of ß3 integrin and NRP1 decreased VEGF-mediated angiogenic responses further than individual inhibition of these receptors. We also show that avß3 integrin can associate with NRP1 in a VEGF-dependent fashion. Our data suggest that ß3 integrin may, in part, negatively regulate VEGF signaling by sequestering NRP1 and preventing it from interacting with VEGFR2.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
| Depositing User: | Users 2731 not found. |
| Date Deposited: | 22 Nov 2011 14:02 |
| Last Modified: | 21 May 2024 09:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/35535 |
| DOI: | 10.1074/jbc.M109.030700 |
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