Analysis of epithelial cell shedding and gaps in the intestinal epithelium

Duckworth, Carrie A and Watson, Alastair J ORCID: (2011) Analysis of epithelial cell shedding and gaps in the intestinal epithelium. Methods in Molecular Biology, 763. pp. 105-114. ISSN 1940-6029

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The intestinal barrier is formed by a monolayer of columnar epithelial cells. This barrier is effectively maintained despite the high turnover of epithelial cells in the gut. Defects in the mechanism by which barrier function is maintained are believed to play a central role in the pathogenesis of inflammatory bowel disease (IBD). Proinflammatory cytokines such as TNF-α and IFN-γ are often elevated in inflamed tissue of patients with IBD. In fact, anti-TNF-α therapy is routinely administered to patients with Crohn's disease. We have previously demonstrated that intestinal epithelial cells are shed from the intestine leaving a 'gap' in the epithelium that is able to maintain barrier function. The rate of cell shedding and barrier permeability is substantially increased by the administration of TNF-α. Loss of barrier function at the site of a gap may provide a site of entry for disease-causing bacteria.

Item Type: Article
Uncontrolled Keywords: acriflavine,animals,crohn disease,cyclic amp,dextrans,female,fluorescein,fluorescent dyes,goblet cells,humans,interferon-gamma,intestine, small,isoquinolines,male,mice,mice, inbred c57bl,microscopy, confocal,permeability,tumor necrosis factor-alpha
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User: Users 2731 not found.
Date Deposited: 14 Nov 2011 13:07
Last Modified: 19 Aug 2023 23:58
DOI: 10.1007/978-1-61779-191-8_7


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