mRNA expression profiling reveals conserved and non-conserved miR-140 targets

Nicolas, Francisco Esteban, Pais, Helio, Schwach, Frank, Lindow, Morten, Kauppinen, Sakari, Moulton, Vincent and Dalmay, Tamas (2011) mRNA expression profiling reveals conserved and non-conserved miR-140 targets. RNA Biology, 8 (4). pp. 607-615. ISSN 1547-6286

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Abstract

microRNAs are non-coding RNAs that regulate gene expression. A significant proportion of microRNAs is perfectly conserved across the vertebrate clade, including miR-140, which is specifically expressed in cartilage. Although it has been computationally predicted that a large majority of microRNA targets are conserved, experimental evidence for this hypothesis remains scarce. In this work we use mRNA expression profiles obtained after manipulation of miR-140 activity levels in human and chicken primary chondrocytes to explore the extent of miR-140 target conservation. Our data suggest that miR-140 has a large number of targets conserved between human and chicken and we validate one of these, BMP2. However, we also found a significant number of non-conserved targets in the two species. In addition, we found that a commercially available scrambled siRNA, which is regularly used as a negative control, regulate the accumulation of many genes.

Item Type: Article
Faculty \ School: Faculty of Science > School of Computing Sciences
Faculty of Science > School of Biological Sciences
Depositing User: Users 2731 not found.
Date Deposited: 18 Oct 2011 13:39
Last Modified: 21 Apr 2020 16:50
URI: https://ueaeprints.uea.ac.uk/id/eprint/35048
DOI: 10.4161/rna.8.4.15390

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