The reaction of flavonoid metabolites with peroxynitrite

Pollard, Susan E., Kuhnle, Gunter G. C., Vauzour, David ORCID: https://orcid.org/0000-0001-5952-8756, Vafeiadou, Katerina, Tzounis, Xenofon, Whiteman, Matthew, Rice-Evans, Catherine and Spencer, Jeremy P. E. (2006) The reaction of flavonoid metabolites with peroxynitrite. Biochemical and Biophysical Research Communications, 350 (4). pp. 960-968. ISSN 0006-291X

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Abstract

There is much interest in the bioactivity of in vivo flavonoid metabolites. We report for the first time the hierarchy of reactivity of flavonoid metabolites with peroxynitrite and characterise novel reaction products. O-Methylation of the B-ring catechol containing flavonoids epicatechin and quercetin, and O-glucuronidation of all flavonoids reduced their reactivity with peroxynitrite. The reaction of the flavanones hesperetin and naringenin and their glucuronides resulted in the formation of multiple mono-nitrated and nitrosated products. In contrast, the catechol-containing flavonoids epicatechin and quercetin yielded oxidation products which when trapped with glutathione led to the production of glutathionyl-conjugates. However, the O-methylated metabolites of epicatechin yielded both mono- and di-nitrated products and nitrosated metabolites. The 3'-O-methyl metabolite of quercetin also yielded a nitrosated species, although its counterpart 4'-O-methyl quercetin yielded only oxidation products. Such products may represent novel metabolic products in vivo and may also express cellular activity.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	David Vauzour
Date Deposited:	14 Apr 2012 20:31
Last Modified:	06 Jun 2024 14:40
URI:	https://ueaeprints.uea.ac.uk/id/eprint/34830
DOI:	10.1016/j.bbrc.2006.09.131

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