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Strube, Thorsten, Schiemann, Olav, Macmillan, Fraser 
ORCID: https://orcid.org/0000-0002-2410-4790, Prisner, Thomas and Engels, Joachim W.
(2001)
A new facile method for spin-labeling of oligonucleotides.
Nucleosides, Nucleotides & Nucleic Acids, 20 (4-7).
pp. 1271-1274.
ISSN 1525-7770
Abstract
A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Light and Energy |
| Depositing User: | Rachel Smith |
| Date Deposited: | 18 Jul 2011 15:55 |
| Last Modified: | 24 Sep 2024 10:25 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/33888 |
| DOI: | 10.1081/NCN-100002534 |
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