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Dhanji, Hiran, Patel, Rajesh, Wall, Robert, Doumith, Michel, Patel, Bharat, Hope, Russell, Livermore, David M. 
ORCID: https://orcid.org/0000-0002-9856-3703 and Woodford, Neil
(2011)
Variation in the genetic environments of blaCTX-M-15 in Escherichia coli from the faeces of travellers returning to the United Kingdom.
Journal of Antimicrobial Chemotherapy, 66 (5).
pp. 1005-1012.
ISSN 0305-7453
Abstract
Objective The genetic surroundings of blaCTX-M-15 in Escherichia coli recovered from faeces of travellers returning to the UK from overseas were compared with those among established UK strains to provide further insights into the spread of blaCTX-M-15 in the UK. Methods From August 2006 to January 2008, 1031 faecal specimens were collected at the North West London NHS Trust from general practice patients with a clinical history of diarrhoea following recent international travel. Cefuroxime-resistant E. coli were isolated on cystine-lactose-electrolyte deficient agar and those that produced extended-spectrum ß-lactamases (ESBLs) were identified by double disc synergy test (DDST). The molecular environments surrounding blaCTX-M-15 were investigated by PCR, DNA sequencing, gene cloning and northern blotting. Results 182/1031 (18%) E. coli isolated from returning travellers gave a positive DDST, and were confirmed by PCR to produce CTX-M ESBLs; 174 (96%) had blaCTX-M-15, including 21 belonging to clone ST131. Among these 174 isolates, the environment upstream of blaCTX-M-15 consisted of either: (i) an intact ISEcp1 (n?=?108); (ii) various lengths of truncated ISEcp1 (n?=?58); or (iii) a 24 bp remnant of ISEcp1 (n?=?8). Two different promoters were found to transcribe blaCTX-M-15, resulting in different levels of cephalosporin resistance. Conclusion E. coli with CTX-M-15 ESBL from returning travellers harboured previously seen UK blaCTX-M-15 genetic environments (intact or 24 bp remnant of ISEcp1) as well as blaCTX-M-15 genetic environments previously unseen in the UK (various lengths of truncated ISEcp1), which suggest overseas acquisition and highlight the difficulty of control in a time of population mobility and travel.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | anti-bacterial agents,blotting, northern,cefuroxime,cloning, molecular,culture media,dna, bacterial,diarrhea,escherichia coli,feces,humans,london,microbial sensitivity tests,polymerase chain reaction,sequence analysis, dna,travel,beta-lactamases,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 13 Jul 2011 10:59 |
| Last Modified: | 22 Nov 2022 14:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/33598 |
| DOI: | 10.1093/jac/dkr041 |
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