Variation in the genetic environments of blaCTX-M-15 in Escherichia coli from the faeces of travellers returning to the United Kingdom

Dhanji, Hiran, Patel, Rajesh, Wall, Robert, Doumith, Michel, Patel, Bharat, Hope, Russell, Livermore, David M and Woodford, Neil (2011) Variation in the genetic environments of blaCTX-M-15 in Escherichia coli from the faeces of travellers returning to the United Kingdom. Journal of Antimicrobial Chemotherapy, 66 (5). pp. 1005-1012. ISSN 0305-7453

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Abstract

Objective The genetic surroundings of blaCTX-M-15 in Escherichia coli recovered from faeces of travellers returning to the UK from overseas were compared with those among established UK strains to provide further insights into the spread of blaCTX-M-15 in the UK. Methods From August 2006 to January 2008, 1031 faecal specimens were collected at the North West London NHS Trust from general practice patients with a clinical history of diarrhoea following recent international travel. Cefuroxime-resistant E. coli were isolated on cystine-lactose-electrolyte deficient agar and those that produced extended-spectrum ß-lactamases (ESBLs) were identified by double disc synergy test (DDST). The molecular environments surrounding blaCTX-M-15 were investigated by PCR, DNA sequencing, gene cloning and northern blotting. Results 182/1031 (18%) E. coli isolated from returning travellers gave a positive DDST, and were confirmed by PCR to produce CTX-M ESBLs; 174 (96%) had blaCTX-M-15, including 21 belonging to clone ST131. Among these 174 isolates, the environment upstream of blaCTX-M-15 consisted of either: (i) an intact ISEcp1 (n?=?108); (ii) various lengths of truncated ISEcp1 (n?=?58); or (iii) a 24 bp remnant of ISEcp1 (n?=?8). Two different promoters were found to transcribe blaCTX-M-15, resulting in different levels of cephalosporin resistance. Conclusion E. coli with CTX-M-15 ESBL from returning travellers harboured previously seen UK blaCTX-M-15 genetic environments (intact or 24 bp remnant of ISEcp1) as well as blaCTX-M-15 genetic environments previously unseen in the UK (various lengths of truncated ISEcp1), which suggest overseas acquisition and highlight the difficulty of control in a time of population mobility and travel.

Item Type: Article
Uncontrolled Keywords: anti-bacterial agents,blotting, northern,cefuroxime,cloning, molecular,culture media,dna, bacterial,diarrhea,escherichia coli,feces,humans,london,microbial sensitivity tests,polymerase chain reaction,sequence analysis, dna,travel,beta-lactamases
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Related URLs:
Depositing User: Rhiannon Harvey
Date Deposited: 13 Jul 2011 10:59
Last Modified: 21 Apr 2020 16:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/33598
DOI: 10.1093/jac/dkr041

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