Variation in the genetic environments of blaCTX-M-15 in Escherichia coli from the faeces of travellers returning to the United Kingdom

Dhanji, Hiran, Patel, Rajesh, Wall, Robert, Doumith, Michel, Patel, Bharat, Hope, Russell, Livermore, David M. and Woodford, Neil (2011) Variation in the genetic environments of blaCTX-M-15 in Escherichia coli from the faeces of travellers returning to the United Kingdom. Journal of Antimicrobial Chemotherapy, 66 (5). pp. 1005-1012. ISSN 0305-7453

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Abstract

Objective The genetic surroundings of blaCTX-M-15 in Escherichia coli recovered from faeces of travellers returning to the UK from overseas were compared with those among established UK strains to provide further insights into the spread of blaCTX-M-15 in the UK. Methods From August 2006 to January 2008, 1031 faecal specimens were collected at the North West London NHS Trust from general practice patients with a clinical history of diarrhoea following recent international travel. Cefuroxime-resistant E. coli were isolated on cystine-lactose-electrolyte deficient agar and those that produced extended-spectrum ß-lactamases (ESBLs) were identified by double disc synergy test (DDST). The molecular environments surrounding blaCTX-M-15 were investigated by PCR, DNA sequencing, gene cloning and northern blotting. Results 182/1031 (18%) E. coli isolated from returning travellers gave a positive DDST, and were confirmed by PCR to produce CTX-M ESBLs; 174 (96%) had blaCTX-M-15, including 21 belonging to clone ST131. Among these 174 isolates, the environment upstream of blaCTX-M-15 consisted of either: (i) an intact ISEcp1 (n?=?108); (ii) various lengths of truncated ISEcp1 (n?=?58); or (iii) a 24 bp remnant of ISEcp1 (n?=?8). Two different promoters were found to transcribe blaCTX-M-15, resulting in different levels of cephalosporin resistance. Conclusion E. coli with CTX-M-15 ESBL from returning travellers harboured previously seen UK blaCTX-M-15 genetic environments (intact or 24 bp remnant of ISEcp1) as well as blaCTX-M-15 genetic environments previously unseen in the UK (various lengths of truncated ISEcp1), which suggest overseas acquisition and highlight the difficulty of control in a time of population mobility and travel.

Item Type:	Article
Uncontrolled Keywords:	anti-bacterial agents,blotting, northern,cefuroxime,cloning, molecular,culture media,dna, bacterial,diarrhea,escherichia coli,feces,humans,london,microbial sensitivity tests,polymerase chain reaction,sequence analysis, dna,travel,beta-lactamases,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health
Depositing User:	Rhiannon Harvey
Date Deposited:	13 Jul 2011 10:59
Last Modified:	06 Feb 2025 01:40
URI:	https://ueaeprints.uea.ac.uk/id/eprint/33598
DOI:	10.1093/jac/dkr041

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