Tools
ToolsHan, Kyung Ho, Choi, Seon Young, Lee, Je Hee, Lee, Hyejon, Shin, Eun Hee, Agtini, Magdarina D., von Seidlein, Lorenz, Ochiai, R. Leon, Clemens, John D., Wain, John, Hahn, Ji-Sook, Lee, Bok Kwon, Song, Manki, Chun, Jongsik and Kim, Dong Wook (2006) Isolation of Salmonella enterica subspecies enterica serovar Paratyphi B dT+, or Salmonella Java, from Indonesia and alteration of the d-tartrate fermentation phenotype by disrupting the ORF STM 3356. Journal of Medical Microbiology, 55 (12). pp. 1661-1665. ISSN 0022-2615
Full text not available from this repository. (Request a copy)Abstract
Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12?:?Hb?:?1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT- (S. Paratyphi B) is the causative agent of paratyphoid fever, and the d-tartrate fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT+ (S. Paratyphi B dT+; formerly called Salmonella Java) causes gastroenteritis. S. Java is currently recognized as an emerging problem worldwide. Twelve dT+ S. Java isolates were collected in Indonesia between 2000 and 2002. One-third of them contained Salmonella genomic island 1 (SGI1), which gives the multidrug-resistant phenotype to the bacteria. In this study, a PCR-based method to detect a single nucleotide difference responsible for the inability to ferment d-tartrate, reported elsewhere, was validated. The d-tartrate fermenting phenotype of S. Java was converted to the non-fermenting phenotype by the disruption of the ORF STM 3356, and the d-tartrate non-fermenting phenotype of the ORF STM 3356-disrupted strain and the dT- reference strain was changed to the dT+ phenotype by complementing ORF STM 3356 in trans. The results show that the dT+ phenotype requires a functional product encoded by STM 3356, and support the use of the PCR-based discrimination method for S. Paratyphi B and S. Java as the standard differentiation method.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018) |
| Depositing User: | Rhiannon Harvey |
| Date Deposited: | 12 Jul 2011 11:11 |
| Last Modified: | 24 Oct 2022 01:36 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/33542 |
| DOI: | 10.1099/jmm.0.46792-0 |
Actions (login required)
 |
View Item |