Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis

Woodford, Neil, Tysall, Luke, Auckland, Cressida, Stockdale, Mark W., Lawson, Andrew J., Walker, Rachel A. and Livermore, David M. ORCID: https://orcid.org/0000-0002-9856-3703 (2002) Detection of oxazolidinone-resistant Enterococcus faecalis and Enterococcus faecium strains by real-time PCR and PCR-restriction fragment length polymorphism analysis. Journal of Clinical Microbiology, 40 (11). pp. 4298-4300. ISSN 0095-1137

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Abstract

A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococcus faecium isolates with a G2576U rRNA mutation. PCR-restriction fragment length polymorphism analysis of ribosomal DNA amplicons with NheI also detected this mutation. Both assays detected isolates heterozygous at this position. Recognition of isolates with what is presently the most frequent oxazolidinone resistance mutation may aid surveillance and individual case management.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health
Depositing User:	Rhiannon Harvey
Date Deposited:	12 Jul 2011 09:59
Last Modified:	12 Jan 2024 01:25
URI:	https://ueaeprints.uea.ac.uk/id/eprint/33525
DOI:	10.1128/JCM.40.11.4298-4300.2002

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