Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp.

Woodford, Neil, Ellington, Matthew J., Coelho, Juliana, Turton, Jane F., Ward, M. Elaina, Brown, Susan, Amyes, Sebastian G. B. and Livermore, David M. ORCID: (2006) Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. International Journal of Antimicrobial Agents, 27 (4). pp. 351-353. ISSN 1872-7913

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Carbapenem resistance in Acinetobacter baumannii is a growing public health concern and is most often mediated by OXA carbapenemases. We describe a novel multiplex polymerase chain reaction (PCR) assay able to detect and distinguish alleles encoding three subgroups of acquired OXA carbapenemases (OXA-23-like, OXA-24-like and OXA-58-like) that are scattered in Acinetobacter spp., and a fourth subgroup, OXA-51-like, which appears to be intrinsic to Acinetobacter baumannii. Isolates belonging to two prevalent UK A. baumannii ‘OXA’ clones (OXA-23 clones 1 and 2) had alleles encoding both an intrinsic OXA-51-like and an acquired OXA-23 enzyme, whereas isolates of the ‘SE clone’ had only an intrinsic blaOXA-51-like allele. Genes encoding OXA-58 were detected (with blaOXA-51-like) in a cluster of related isolates from a single hospital. This simple assay will assist in monitoring the mechanisms responsible for carbapenem resistance in Acinetobacter spp.

Item Type: Article
Uncontrolled Keywords: sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Rhiannon Harvey
Date Deposited: 11 Jul 2011 10:40
Last Modified: 23 Oct 2022 08:30
DOI: 10.1016/j.ijantimicag.2006.01.004

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