A human lens model of cortical cataract: Ca2+-induced protein loss, vimentin cleavage and opacification

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Sanderson, Julie, Marcantonio, Julia M. and Duncan, George (2000) A human lens model of cortical cataract: Ca2+-induced protein loss, vimentin cleavage and opacification. Investigative Ophthalmology & Visual Science, 41 (8). pp. 2255-2261. ISSN 0146-0404

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Abstract

PURPOSE. Cortical cataract in humans is associated with Ca2+ overload and protein loss, and although animal models of cataract have implicated Ca2+-activated proteases in this process, it remains to be determined whether the human lens responds in this manner to conditions of Ca2+ overload. The purpose of these experiments was to investigate Ca2+-induced opacification and proteolysis in the organ-cultured human lens. METHODS. Donor human lenses were cultured in Eagle's minimum essential medium (EMEM) for up to 14 days. The Ca2+ ionophore ionomycin was used to induce a Ca2+ overload. Lenses were loaded with [H-3]-amino acids for 48 hours. After a 24-hour control efflux period, lenses were cultured in control EMEM (Ca2+ 1.8 mM), EMEM + 5 mu M ionomycin, or EMEM + 5 mu M ionomycin + 5 mM EGTA (Ca2+

Item Type: Article
Uncontrolled Keywords: transglutaminase,calpain,senile cataracts,light-scattering,proteolysis,calcium-induced opacification
Faculty \ School: Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups: Faculty of Science > Research Groups > Molecular and Tissue Pharmacology
Faculty of Science > Research Groups > Pharmaceutical Cell Biology (former - to 2017)
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Depositing User: Rachel Smith
Date Deposited: 08 Jun 2011 15:36
Last Modified: 24 Sep 2024 10:28
URI: https://ueaeprints.uea.ac.uk/id/eprint/32081
DOI:

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