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Taguchi, T., Itou, K., Ebizuka, Y., Malpartida, F., Hopwood, D. A., Surti, C. M., Booker-Milburn, K. I., Stephenson, G. R. 
ORCID: https://orcid.org/0000-0003-1487-9178 and Ichinose, K.
(2000)
Chemical characterisation of disruptants of the Streptomyces coelicolor A3(2) actVI genes involved in actinorhodin biosynthesis.
Journal of Antibiotics, 53 (2).
pp. 144-152.
ISSN 0021-8820
Abstract
The actVI genetic region of Streptomyces coelicolor A3(2) is part of the biosynthetic gene cluster of actinorhodin (ACT), the act cluster, consisting of six ORFs: ORFB, ORFA, ORF1, ORF2, ORF3, ORF4. A newly devised method of ACT detection with a combination of HPLC and LC/MS was applied to the analysis of the disruptants of each ORE ACT was produced by those of ORFB, ORFA, ORF3, and ORF4. Instead of ACT, the ORF1 disruptant produced 3,8dihydroxy-1-methylanthraquinone-2-carboxylic acid (DMAC) and aloesaponarin II as shunt products. The ORF2 disruptant gave 4-dihydro-9-hydrsxy-1-methyl-10-oxo-3-H-naphtho-[2,3-c]-pyran-3-(S)-acet ic acid, (S)-DNPA. These results support our previous proposal for stereospecific pyran ring formation in the biosynthesis of ACT, most importantly suggesting that the actVI-ORF2 product would recognize (S)-DNPA as a substrate for stereospecific reduction at C-15. The disruptant of ORFA produced (S)-DNPA together with ACT, suggesting that actVI-ORFA might play a role such as stabilising the multicomponent, type II PKS complex.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry |
| UEA Research Groups: | Faculty of Science > Research Groups > Synthetic Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Materials and Catalysis |
| Depositing User: | Rachel Smith |
| Date Deposited: | 08 Jun 2011 11:15 |
| Last Modified: | 14 Aug 2023 11:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/31996 |
| DOI: | 10.7164/antibiotics.53.144 |
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