Dietary vitamin C down-regulates inflammatory gene expression in apoE4 smokers

Majewicz, Jonathan, Rimbach, Gerald, Proteggente, Anna R., Lodge, John K., Kraemer, Klaus and Minihane, Anne M ORCID: (2005) Dietary vitamin C down-regulates inflammatory gene expression in apoE4 smokers. Biochemical and Biophysical Research Communications, 338 (2). pp. 951-955. ISSN 1090-2104

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The deleterious impact of cigarette smoking on cardiovascular health may be in part attributable to a free radical mediated proinflammatory response in circulating monocytes. In the current investigation, the impact of vitamin C supplementation on monocyte gene expression was determined in apoE4 smokers versus non-smokers. A total of 10 smokers and 11 non-smokers consumed 60 mg/day of vitamin C for four weeks and a fasting blood sample was taken at baseline and post-intervention for the determination of plasma vitamin C and monocyte gene expression profiles using cDNA array and real time PCR. In apoE4 smokers, supplementation resulted in a 43% increase in plasma vitamin C concentrations. Furthermore, a number of genes were differentially expressed more than 2-fold in response to treatment, including a downregulation of the proinflammatory mediators tumor necrosis factor (TNF) β, TNF receptor, neurotrophin-3 growth factor receptor, and monocyte chemoattractant protein 1 receptor. The study has identified a number of molecular mechanisms underlying the benefit of vitamin C supplementation in smokers.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Rhiannon Harvey
Date Deposited: 01 Jun 2011 08:06
Last Modified: 17 Feb 2023 16:30
DOI: 10.1016/j.bbrc.2005.10.029

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