Influence of apolipoprotein E genotype and dietary alpha-tocopherol on redox status and C-reactive protein levels in apolipoprotein E3 and E4 targeted replacement mice

Jofre-Monseny, Laia, Huebbe, Patricia, Stange, Inken, Boesch-Saadatmandi, Christine, Frank, Jan, Jackson, Kim, Minihane, Anne-Marie ORCID: https://orcid.org/0000-0001-9042-4226 and Rimbach, Gerald (2008) Influence of apolipoprotein E genotype and dietary alpha-tocopherol on redox status and C-reactive protein levels in apolipoprotein E3 and E4 targeted replacement mice. British Journal of Nutrition, 100 (1). pp. 44-53. ISSN 0007-1145

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Abstract

The molecular basis of the positive association between apoE4 genotype and CVD remains unclear. There is direct in vitro evidence indicating that apoE4 is a poorer antioxidant relative to the apoE3 isoform, with some indirect in vivo evidence also available. Therefore it was hypothesised that apoE4 carriers may benefit from alpha-tocopherol (alpha-Toc) supplementation. Targeted replacement mice expressing the human apoE3 and apoE4 were fed with a diet poor (0 mg/kg diet) or rich (200 mg/kg diet) in alpha-Toc for 12 weeks. Neither apoE genotype nor dietary alpha-Toc exerted any effects on the antioxidant defence system, including glutathione, catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase activities. In addition, no differences were observed in mitogen-induced lymphocyte proliferation. alpha-Toc concentrations were modestly higher in plasma and lower in tissues of apoE4 compared with apoE3 mice, with the greatest differences evident in the lung, suggesting that an apoE4 genotype may reduce alpha-Toc delivery to tissues. A tendency towards increased plasma F2-isoprostanes in apoE4 mice was observed, while liver thiobarbituric acid-reactive substances did not differ between apoE3 and apoE4 mice. In addition, C-reactive protein (CRP) concentrations were reduced in apoE4 mice indicating that this positive effect on CRP may in part negate the increased CVD risk associated with an apoE4 genotype.

Item Type:	Article
Uncontrolled Keywords:	animals,antioxidants,apolipoprotein e3,apolipoprotein e4,apolipoproteins e,biological markers,c-reactive protein,diet,female,glutathione,humans,lymphocyte activation,mice,mitogens,oxidation-reduction,oxidative stress,spleen,alpha-tocopherol
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Depositing User:	Rhiannon Harvey
Date Deposited:	31 May 2011 15:17
Last Modified:	19 Oct 2023 00:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/31630
DOI:	10.1017/S000711450788634X

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