Name that microbe: Rapid identification of taxa responsible for individual fragments in fingerprints of microbial community structure

Grant, A and Ogilvie, LA (2004) Name that microbe: Rapid identification of taxa responsible for individual fragments in fingerprints of microbial community structure. Molecular Ecology Notes, 4 (1). pp. 133-136.

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Abstract

Polymerase chain reaction (PCR)-based ‘fingerprinting’ methods, such as Terminal restriction fragment length polymorphism, Length Heterogeneity-Polymerase Chain Reaction (LH-PCR) and Automated Ribosomal Intergenic Spacer Analysis (ARISA) make possible quantitative studies of microbial community structure and dynamics. Here we outline a strategy for the rapid and cost-effective isolation of 16S clones corresponding to particular fragment sizes in a fingerprint, based on applying the fingerprinting method to pools of colonies from a clone library. This allows the definitive identification of taxa responsible for the most important bands in the community fingerprint from a full 16S sequence. It offers significant advantages over random selection of clones and removes a significant barrier to the use of these methods.

Item Type: Article
Faculty \ School: Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: Rosie Cullington
Date Deposited: 20 May 2011 09:38
Last Modified: 12 Jul 2020 23:40
URI: https://ueaeprints.uea.ac.uk/id/eprint/31150
DOI: 10.1111/j.1471-8286.2004.00590.x

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