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Grant, Alastair 
ORCID: https://orcid.org/0000-0002-1147-2375 and Ogilvie, Lesley Ann
(2004)
Name that microbe: Rapid identification of taxa responsible for individual fragments in fingerprints of microbial community structure.
Molecular Ecology Notes, 4 (1).
pp. 133-136.
Abstract
Polymerase chain reaction (PCR)-based ‘fingerprinting’ methods, such as Terminal restriction fragment length polymorphism, Length Heterogeneity-Polymerase Chain Reaction (LH-PCR) and Automated Ribosomal Intergenic Spacer Analysis (ARISA) make possible quantitative studies of microbial community structure and dynamics. Here we outline a strategy for the rapid and cost-effective isolation of 16S clones corresponding to particular fragment sizes in a fingerprint, based on applying the fingerprinting method to pools of colonies from a clone library. This allows the definitive identification of taxa responsible for the most important bands in the community fingerprint from a full 16S sequence. It offers significant advantages over random selection of clones and removes a significant barrier to the use of these methods.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Environmental Sciences |
| UEA Research Groups: | Faculty of Science > Research Centres > Centre for Ecology, Evolution and Conservation Faculty of Science > Research Groups > Environmental Biology Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Groups > Resources, Sustainability and Governance (former - to 2018) Faculty of Science > Research Groups > Marine and Atmospheric Sciences (former - to 2017) |
| Depositing User: | Rosie Cullington |
| Date Deposited: | 20 May 2011 09:38 |
| Last Modified: | 06 Mar 2023 16:31 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/31150 |
| DOI: | 10.1111/j.1471-8286.2004.00590.x |
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