Stimulation of stress-activated but not mitogen-activated protein kinases by tumour necrosis factor receptor subtypes in airway smooth muscle

McFarlane, Shona M., Jupp, Orla J., Cobban, Hannah J., Hunter, Irene, Anderson, Helen M., Vandenabeele, Peter, Nixon, Graeme F. and MacEwan, David J. (2001) Stimulation of stress-activated but not mitogen-activated protein kinases by tumour necrosis factor receptor subtypes in airway smooth muscle. Biochemical Pharmacology, 61 (6). pp. 749-759. ISSN 0006-2952

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Abstract

The multifunctional cytokine tumour necrosis factor-alpha (TNF) displays many physiological effects in a variety of tissues, especially proliferative and cytotoxic actions in immunological cells. Recently, we uncovered an important new mechanism by which TNF can sensitise airway smooth muscle (ASM) to a fixed intracellular Ca2+ concentration which in vivo would produce a marked hypercontractility of the airways. Here, we report that both 50-60 kDa type I TNFR (TNFR1) and 70-80 kDa type II TNFR (TNFR2) receptor subtypes were expressed in ASM cells and selectively activated the stress kinases, c-Jun N-terminal kinase and p38 mitogen-activated protein kinase (p38 MAPK), However, TNF caused no activation of p42/p44 MAPK or cytosolic phospholipase A(2) activity. In contrast, TNF stimulation of the TNFR1, but not the TNFR2, elicited nuclear factor-kappaB transcription factor function, a species known to be important in mediation of certain inflammatory cellular responses. This is the first report of TNF receptor subtypes in ASM cells causing selective kinase activation, which may prove important in therapeutic strategies for treating immune airway disorders such as chronic obstructive pulmonary disease and asthma. (C) 2001 Elsevier Science Inc. All rights reserved.

Item Type:	Article
Uncontrolled Keywords:	cell-death,smooth muscle,tnf,airways,phosphorylation,factor-alpha,pathway,cytosolic phospholipase a(2),asthma,fusion protein,tumour necrosis factor,human platelets,nuclear-envelope,factor-kappa-b,hypercontractility,signalling,signaling
Faculty \ School:	Faculty of Science > School of Pharmacy
Depositing User:	Rachel Smith
Date Deposited:	23 May 2011 14:03
Last Modified:	08 May 2024 10:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/31038
DOI:	10.1016/S0006-2952(01)00530-5

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