Polymerase chain reaction in the detection of an 'outbreak' of asymptomatic viral infections in a community birth cohort in south India

Gladstone, B. P., Iturriza-Gomara, M., Ramani, S., Monica, B., Banerjee, I., Brown, D. W., Gray, J. J., Muliyil, J. and Kang, G. (2008) Polymerase chain reaction in the detection of an 'outbreak' of asymptomatic viral infections in a community birth cohort in south India. Epidemiology and Infection, 136 (3). pp. 399-405. ISSN 0950-2688

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Abstract

Asymptomatic enteric infections are important where sequelae or protection from subsequent illness is an outcome measure. The use of reverse transcription-polymerase chain reaction (RT-PCR) to identify asymptomatic enteric infections in a birth cohort followed for rotaviral infections in a south Indian urban slum is reported. Of 1191 non-diarrhoeal samples from 371 children collected in May-June 2003, 22 (1.9%) were positive by ELISA. A total of 147 (40.6%) of 362 samples tested by VP6 RT-PCR were positive. In those samples that could be typed, a high diversity of G types including G1, G2, G4, G8, G9 and G10, and a high proportion (34.4%) of mixed infections were detected. Noroviruses were identified in 6/28 (21.4%) samples tested. The identification of infections undetectable by conventional techniques indicates the importance of the use of sensitive diagnostic techniques in research studies. Asymptomatically infected children may also act as a source of infection for other susceptible hosts.

Item Type:	Article
Uncontrolled Keywords:	sdg 11 - sustainable cities and communities ,/dk/atira/pure/sustainabledevelopmentgoals/sustainable_cities_and_communities
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User:	Rhiannon Harvey
Date Deposited:	18 May 2011 13:37
Last Modified:	24 Sep 2024 09:32
URI:	https://ueaeprints.uea.ac.uk/id/eprint/30817
DOI:	10.1017/S0950268807008709

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