Ultrasound-enhanced transgene expression in vascular cells is not dependent upon cavitation-induced free radicals

Lawrie, Allan, Brisken, Axel F, Francis, Sheila E, Wyllie, David, Kiss-Toth, Endre, Qwarnstrom, Eva E, Dower, Stephen K, Crossman, David C. and Newman, Christopher M (2003) Ultrasound-enhanced transgene expression in vascular cells is not dependent upon cavitation-induced free radicals. Ultrasound in Medicine & Biology, 29 (10). pp. 1453-1461. ISSN 0301-5629

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Abstract

Although acoustic cavitation is clearly important in ultrasound (US)-enhanced gene delivery (UEGD), the relative importance of mechanical and sonochemical (free radical) bioeffects remains unclear, as does the mechanism of gene delivery at the cellular level. Porcine vascular smooth muscle cells (VSMC) were transfected with luciferase or green fluorescent protein (GFP) plasmid +/- pulsed 956 kHz US (2.0 mechanical index (MI), 128 W cm(-2) spatial peak pulse average intensity, ISPPA) for 60 s, in the presence or absence of 20 mM cysteamine or N-acetyl-L-cysteine. Both compounds effectively scavenged free radical production following US, leaving unaffected the 50- to 100-fold enhancements in luciferase expression seen in US-treated VSMC. US exposure enhanced plasmid uptake (25 +/- 4.6 vs. 3 +/- 1.9 cells/field, n=4, psevenfold) and average fluorescence intensity (>sixfold) of GFP-transfected cells. UEGD is not dependent upon cavitation-induced free radical generation and has potential for use with a wide range of therapeutic transgenes.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Norwich Clinical Trials Unit
Depositing User:	Rhiannon Harvey
Date Deposited:	17 May 2011 15:20
Last Modified:	23 Oct 2022 18:32
URI:	https://ueaeprints.uea.ac.uk/id/eprint/30693
DOI:	10.1016/S0301-5629(03)01032-9

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