Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair

Bouter, Anthony, Gounou, Céline, Bérat, Rémi, Tan, Sisareuth, Gallois, Bernard, Granier, Thierry, d'Estaintot, Béatrice Langlois, Pöschl, Ernst, Brachvogel, Bent and Brisson, Alain R. (2011) Annexin-A5 assembled into two-dimensional arrays promotes cell membrane repair. Nature Communications, 2. ISSN 2041-1723

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Abstract

Eukaryotic cells possess a universal repair machinery that ensures rapid resealing of plasma membrane disruptions. Before resealing, the torn membrane is submitted to considerable tension, which functions to expand the disruption. Here we show that annexin-A5 (AnxA5), a protein that self-assembles into two-dimensional (2D) arrays on membranes upon Ca2+ activation, promotes membrane repair. Compared with wild-type mouse perivascular cells, AnxA5-null cells exhibit a severe membrane repair defect. Membrane repair in AnxA5-null cells is rescued by addition of AnxA5, which binds exclusively to disrupted membrane areas. In contrast, an AnxA5 mutant that lacks the ability of forming 2D arrays is unable to promote membrane repair. We propose that AnxA5 participates in a previously unrecognized step of the membrane repair process: triggered by the local influx of Ca2+, AnxA5 proteins bind to torn membrane edges and form a 2D array, which prevents wound expansion and promotes membrane resealing.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Cells and Tissues
Depositing User:	Users 2731 not found.
Date Deposited:	16 May 2011 11:31
Last Modified:	21 Apr 2023 23:51
URI:	https://ueaeprints.uea.ac.uk/id/eprint/30578
DOI:	10.1038/ncomms1270

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