The relationship between redox enzyme activity and electrochemical potential—cellular and mechanistic implications from protein film electrochemistry

Gates, Andrew J., Kemp, Gemma L., To, Chun Yip, Mann, James, Marritt, Sophie J., Mayes, Andrew G., Richardson, David J. and Butt, Julea N. (2011) The relationship between redox enzyme activity and electrochemical potential—cellular and mechanistic implications from protein film electrochemistry. Physical Chemistry Chemical Physics, 13 (17). pp. 7720-7731. ISSN 1463-9076

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Abstract

In protein film electrochemistry a redox protein of interest is studied as an electroactive film adsorbed on an electrode surface. For redox enzymes this configuration allows quantification of the relationship between catalytic activity and electrochemical potential. Considered as a function of enzyme environment, i.e., pH, substrate concentration etc., the activity–potential relationship provides a fingerprint of activity unique to a given enzyme. Here we consider the nature of the activity–potential relationship in terms of both its cellular impact and its origin in the structure and catalytic mechanism of the enzyme. We propose that the activity–potential relationship of a redox enzyme is tuned to facilitate cellular function and highlight opportunities to test this hypothesis through computational, structural, biochemical and cellular studies.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Chemistry
Depositing User: Rachel Smith
Date Deposited: 18 Apr 2011 11:54
Last Modified: 17 Sep 2020 23:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/29544
DOI: 10.1039/c0cp02887h

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