The relationship between redox enzyme activity and electrochemical potential—cellular and mechanistic implications from protein film electrochemistry

Gates, Andrew J. ORCID: https://orcid.org/0000-0002-4594-5038, Kemp, Gemma L., To, Chun Yip, Mann, James, Marritt, Sophie J., Mayes, Andrew G., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832 and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 (2011) The relationship between redox enzyme activity and electrochemical potential—cellular and mechanistic implications from protein film electrochemistry. Physical Chemistry Chemical Physics, 13 (17). pp. 7720-7731. ISSN 1463-9076

Preview

PDF (Gates_et_al_PCCP_2011.pdf) Download (3MB) | Preview

Abstract

In protein film electrochemistry a redox protein of interest is studied as an electroactive film adsorbed on an electrode surface. For redox enzymes this configuration allows quantification of the relationship between catalytic activity and electrochemical potential. Considered as a function of enzyme environment, i.e., pH, substrate concentration etc., the activity–potential relationship provides a fingerprint of activity unique to a given enzyme. Here we consider the nature of the activity–potential relationship in terms of both its cellular impact and its origin in the structure and catalytic mechanism of the enzyme. We propose that the activity–potential relationship of a redox enzyme is tuned to facilitate cellular function and highlight opportunities to test this hypothesis through computational, structural, biochemical and cellular studies.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017) Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Materials and Catalysis Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory
Depositing User:	Rachel Smith
Date Deposited:	18 Apr 2011 11:54
Last Modified:	15 May 2023 00:06
URI:	https://ueaeprints.uea.ac.uk/id/eprint/29544
DOI:	10.1039/c0cp02887h

Downloads

Actions (login required)

View Item