Excited state reactions in fluorescent proteins

Meech, Stephen R. ORCID: https://orcid.org/0000-0001-5561-2782 (2009) Excited state reactions in fluorescent proteins. Chemical Society Reviews, 38 (10). pp. 2922-2934. ISSN 1460-4744

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Abstract

The green fluorescent protein is a key technology in bioimaging. In this critical review, we consider how its various applications can be tailored from knowledge of the excited state chemistry. The photophysics of the basic chromophore in solution are described in detail, and the dominant radiationless decay mechanism is characterised. The quite different photophysics of wild type GFP are described next. The unique excited state proton transfer reaction observed can be used to model proton transfer processes in proteins. Examples where the proton transfer is blocked, or redirected to occur over a low short barrier H-bond are discussed. Finally the photophysics underlying the new generation of photochemically active fluorescent proteins are discussed (155 references).

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Chemistry (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Physical and Analytical Chemistry (former - to 2017) Faculty of Science > Research Groups > Centre for Photonics and Quantum Science
Depositing User:	Rachel Smith
Date Deposited:	14 Apr 2011 10:27
Last Modified:	24 Sep 2024 09:24
URI:	https://ueaeprints.uea.ac.uk/id/eprint/27012
DOI:	10.1039/b820168b

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