Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells

Sharp, Paul, Tandy, Sarah, Yamaji, Sachie, Tennant, Jason, Williams, Mark and Singh Srai, Surjit Kaila (2002) Rapid regulation of divalent metal transporter (DMT1) protein but not mRNA expression by non-haem iron in human intestinal Caco-2 cells. FEBS Letters, 510 (1-2). pp. 71-76. ISSN 1873-3468

Full text not available from this repository. (Request a copy)

Abstract

A divalent metal transporter, DMT1, located on the apical membrane of intestinal enterocytes is the major pathway for the absorption of dietary non-haem iron. Using human intestinal Caco-2 TC7 cells, we have shown that iron uptake and DMT1 protein in the plasma membrane were significantly decreased by exposure to high iron for 24 h, in a concentration-dependent manner, whereas whole cell DMT1 protein abundance was unaltered. This suggests that part of the response to high iron involved redistribution of DMT1 between the cytosol and cell membrane. These events preceded changes in DMT1 mRNA, which was only decreased following 72 h exposure to high iron.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
Depositing User:	Users 2731 not found.
Date Deposited:	07 Mar 2011 12:20
Last Modified:	15 Dec 2022 01:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/25692
DOI:	10.1016/S0014-5793(01)03225-2

Actions (login required)

View Item