High throughput sequencing of microRNAs in chicken somites

Rathjen, Tina, Pais, Helio, Sweetman, Dylan, Moulton, Vincent ORCID: https://orcid.org/0000-0001-9371-6435, Munsterberg, Andrea ORCID: https://orcid.org/0000-0002-4577-4240 and Dalmay, Tamas ORCID: https://orcid.org/0000-0003-1492-5429 (2009) High throughput sequencing of microRNAs in chicken somites. FEBS Letters, 583 (9). pp. 1422-1426. ISSN 1873-3468

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Abstract

High throughput Solexa sequencing technology was applied to identify microRNAs in somites of developing chicken embryos. We obtained 651 273 reads, from which 340 415 were mapped to the chicken genome representing 1701 distinct sequences. Eighty-five of these were known microRNAs and 42 novel miRNA candidates were identified. Accumulation of 18 of 42 sequences was confirmed by Northern blot analysis. Ten of the 18 sequences are new variants of known miRNAs and eight short RNAs are novel miRNAs. Six of these eight have not been reported by other deep sequencing projects. One of the six new miRNAs is highly enriched in somite tissue suggesting that deep sequencing of other specific tissues has the potential to identify novel tissue specific miRNAs.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Computing Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Computational Biology > Computational biology of RNA (former - to 2018) Faculty of Science > Research Groups > Computational Biology > Phylogenetics (former - to 2018) Faculty of Science > Research Groups > Plant Sciences Faculty of Science > Research Groups > Cells and Tissues Faculty of Science > Research Groups > Computational Biology Faculty of Science > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:36
Last Modified:	15 Jun 2023 15:32
URI:	https://ueaeprints.uea.ac.uk/id/eprint/253
DOI:	10.1016/j.febslet.2009.03.048

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