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ToolsWilmes, Paul, Wexler, Margaret and Bond, Philip L. (2008) Metaproteomics provides functional insight into activated sludge wastewater treatment. PLoS One, 3 (3). ISSN 1932-6203
Full text not available from this repository.Abstract
Background: Through identification of highly expressed proteins from a mixed culture activated sludge system this study provides functional evidence of microbial transformations important for enhanced biological phosphorus removal (EBPR). Methodology/Principal Findings: A laboratory-scale sequencing batch reactor was successfully operated for different levels of EBPR, removing around 25, 40 and 55 mg/l P. The microbial communities were dominated by the uncultured polyphosphate-accumutating organism "Candidatus Accumulibacter phosphatis". When EBPR failed, the sludge was dominated by tetrad-forming a-Proteobacterio. Representative and reproducible 2D gel protein separations were obtained for all sludge samples. 638 protein spots were matched across gels generated from the phosphate removing sludges. 111 of these were excised and 46 proteins were identified using recently available sludge metagenomic sequences. Many of these closely match proteins from "Candidatus Accumulibacter phosphatis" and could be directly linked to the EBPR process. They included enzymes involved inenergy generation, polyhydroxyalkanoate synthesis, glycolysis, gluconeogenesis, glycogen synthesis, glyoxylate/ TCA cycle, fatty acid ß oxidation, fatty acid synthesis and phosphate transport. Several proteins involved in cellular stress response were detected. Conclusions/Significance: Importantly, this study provides direct evidence linking the metabolic activities of "Accumulibacter" to the chemical transformations observed in EBPR. Finally, the results are discussed in relation to current EBPR metabolic models.
| Item Type: | Article |
|---|---|
| Additional Information: | © 2008 Wilmes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Depositing User: | Rosie Cullington |
| Date Deposited: | 26 Feb 2011 12:23 |
| Last Modified: | 23 Oct 2022 06:32 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/24840 |
| DOI: | 10.1371/journal.pone.0001778 |
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