Tools
Tools
Li, Yunhai, Lee, Kee Khoon, Walsh, Sean, Smith, Caroline, Hadingham, Sophie, Sorefan, Karim, Cawley, Gavin C. 
ORCID: https://orcid.org/0000-0002-4118-9095 and Bevan, Michael W.
(2006)
Establishing glucose- and ABA-regulated transcription networks in Arabidopsis by microarray analysis and promoter classification using a Relevance Vector Machine.
Genome Research, 16 (3).
pp. 414-427.
ISSN 1088-9051
Abstract
Establishing transcriptional regulatory networks by analysis of gene expression data and promoter sequences shows great promise. We developed a novel promoter classification method using a Relevance Vector Machine (RVM) and Bayesian statistical principles to identify discriminatory features in the promoter sequences of genes that can correctly classify transcriptional responses. The method was applied to microarray data obtained from Arabidopsis seedlings treated with glucose or abscisic acid (ABA). Of those genes showing >2.5-fold changes in expression level, ~70% were correctly predicted as being up- or down-regulated (under 10-fold cross-validation), based on the presence or absence of a small set of discriminative promoter motifs. Many of these motifs have known regulatory functions in sugar- and ABA-mediated gene expression. One promoter motif that was not known to be involved in glucose-responsive gene expression was identified as the strongest classifier of glucose-up-regulated gene expression. We show it confers glucose-responsive gene expression in conjunction with another promoter motif, thus validating the classification method. We were able to establish a detailed model of glucose and ABA transcriptional regulatory networks and their interactions, which will help us to understand the mechanisms linking metabolism with growth in Arabidopsis. This study shows that machine learning strategies coupled to Bayesian statistical methods hold significant promise for identifying functionally significant promoter sequences.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Computing Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Groups > Data Science and Statistics Faculty of Science > Research Groups > Computational Biology |
| Depositing User: | Vishal Gautam |
| Date Deposited: | 26 May 2011 10:36 |
| Last Modified: | 21 Apr 2023 21:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/21577 |
| DOI: | 10.1101/gr.4237406 |
Actions (login required)
 |
View Item |