Inhibitory effect of calcium on non-heme iron absorption may be related to translocation of DMT-1 at the apical membrane of enterocytes

Thompson, Ben A V, Sharp, Paul A, Elliott, Ruan and Fairweather-Tait, Susan J (2010) Inhibitory effect of calcium on non-heme iron absorption may be related to translocation of DMT-1 at the apical membrane of enterocytes. Journal of Agricultural and Food Chemistry, 58 (14). pp. 8414-8417. ISSN 0021-8561

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Abstract

Many studies show that calcium reduces iron absorption from single meals, but the underlying mechanism is not known. We tested the hypothesis that calcium alters the expression and/or functionality of iron transport proteins. Differentiated Caco-2 cells were treated with ferric ammonium citrate and calcium chloride, and ferritin, DMT-1, and ferroportin were quantified in whole-cell lysate and cell-membrane fractions. Calcium attenuated the iron-induced increase in cell ferritin levels in a dose-dependent manner; a significant decrease was seen at calcium concentrations of 1.25 and 2.5 mM but was only evident after a 16-24 h incubation period. Calcium and iron treatments decreased DMT-1 protein in Caco-2 cell membranes, although total DMT-1 in whole cell lysates was unchanged by either iron or calcium. No change was seen in ferroportin expression. Our data suggest that calcium reduces iron bioavailability by decreasing DMT-1 expression at the apical cell membrane, thereby downregulating iron transport into the cell.

Item Type: Article
Uncontrolled Keywords: biological transport,caco-2 cells,calcium,cation transport proteins,cell membrane,down-regulation,enterocytes,humans,intestinal absorption,iron,protein transport
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
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Depositing User: Rhiannon Harvey
Date Deposited: 17 Feb 2011 12:37
Last Modified: 28 Oct 2019 13:41
URI: https://ueaeprints.uea.ac.uk/id/eprint/21496
DOI: 10.1021/jf101388z

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