Inhibitory effect of calcium on non-heme iron absorption may be related to translocation of DMT-1 at the apical membrane of enterocytes

Thompson, Ben A V, Sharp, Paul A, Elliott, Ruan and Fairweather-Tait, Susan J ORCID: https://orcid.org/0000-0002-1413-5569 (2010) Inhibitory effect of calcium on non-heme iron absorption may be related to translocation of DMT-1 at the apical membrane of enterocytes. Journal of Agricultural and Food Chemistry, 58 (14). pp. 8414-8417. ISSN 0021-8561

Full text not available from this repository. (Request a copy)

Abstract

Many studies show that calcium reduces iron absorption from single meals, but the underlying mechanism is not known. We tested the hypothesis that calcium alters the expression and/or functionality of iron transport proteins. Differentiated Caco-2 cells were treated with ferric ammonium citrate and calcium chloride, and ferritin, DMT-1, and ferroportin were quantified in whole-cell lysate and cell-membrane fractions. Calcium attenuated the iron-induced increase in cell ferritin levels in a dose-dependent manner; a significant decrease was seen at calcium concentrations of 1.25 and 2.5 mM but was only evident after a 16-24 h incubation period. Calcium and iron treatments decreased DMT-1 protein in Caco-2 cell membranes, although total DMT-1 in whole cell lysates was unchanged by either iron or calcium. No change was seen in ferroportin expression. Our data suggest that calcium reduces iron bioavailability by decreasing DMT-1 expression at the apical cell membrane, thereby downregulating iron transport into the cell.

Item Type:	Article
Uncontrolled Keywords:	biological transport,caco-2 cells,calcium,cation transport proteins,cell membrane,down-regulation,enterocytes,humans,intestinal absorption,iron,protein transport
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Depositing User:	Rhiannon Harvey
Date Deposited:	17 Feb 2011 12:37
Last Modified:	19 Oct 2023 00:37
URI:	https://ueaeprints.uea.ac.uk/id/eprint/21496
DOI:	10.1021/jf101388z

Actions (login required)

View Item