Reductive activation of nitrate reductases

Field, Sarah J., Thornton, Nicholas P., Anderson, Lee J., Gates, Andrew J. ORCID: https://orcid.org/0000-0002-4594-5038, Reilly, Ann, Jepson, Brian J. N., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, George, Simon J., Cheesman, Myles R. and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 (2005) Reductive activation of nitrate reductases. Dalton Transactions (21). pp. 3580-3586. ISSN 1477-9234

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Abstract

Protein film voltammetry of Paracoccus pantotrophus respiratory nitrate reductase (NarGH) and Synechococcus elongatus assimilatory nitrate reductase (NarB) shows that reductive activation of these enzymes may be required before steady state catalysis is observed. For NarGH complementary spectroscopic studies suggest a structural context for the activation. Catalytic protein film voltammetry at a range of temperatures has allowed quantitation of the activation energies for nitrate reduction. For NarGH with an operating potential of ca. 0.05 V the activation energy of ca. 35 kJ mol-1 is over twice that measured for NarB whose operating potential is ca. -0.35 V.

Item Type: Article
Faculty \ School: Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Science > Research Groups > Organisms and the Environment
Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017)
Faculty of Science > Research Groups > Chemistry of Light and Energy
Faculty of Science > Research Groups > Chemistry of Life Processes
Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Faculty of Science > Research Groups > Energy Materials Laboratory
Depositing User: Rachel Smith
Date Deposited: 17 Feb 2011 14:37
Last Modified: 17 May 2023 00:00
URI: https://ueaeprints.uea.ac.uk/id/eprint/21411
DOI: 10.1039/b505530j

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