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Field, Sarah J., Thornton, Nicholas P., Anderson, Lee J., Gates, Andrew J. 
ORCID: https://orcid.org/0000-0002-4594-5038, Reilly, Ann, Jepson, Brian J. N., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, George, Simon J., Cheesman, Myles R. and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226
(2005)
Reductive activation of nitrate reductases.
Dalton Transactions (21).
pp. 3580-3586.
ISSN 1477-9234
Abstract
Protein film voltammetry of Paracoccus pantotrophus respiratory nitrate reductase (NarGH) and Synechococcus elongatus assimilatory nitrate reductase (NarB) shows that reductive activation of these enzymes may be required before steady state catalysis is observed. For NarGH complementary spectroscopic studies suggest a structural context for the activation. Catalytic protein film voltammetry at a range of temperatures has allowed quantitation of the activation energies for nitrate reduction. For NarGH with an operating potential of ca. 0.05 V the activation energy of ca. 35 kJ mol-1 is over twice that measured for NarB whose operating potential is ca. -0.35 V.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory |
| Depositing User: | Rachel Smith |
| Date Deposited: | 17 Feb 2011 14:37 |
| Last Modified: | 17 May 2023 00:00 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/21411 |
| DOI: | 10.1039/b505530j |
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