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Blumenschein, Tharin M. A 
ORCID: https://orcid.org/0000-0002-4932-5178, Stone, Deborah B., Fletterick, Robert J., Mendelson, Robert A. and Sykes, Brian D.
(2006)
Dynamics of the C-terminal region of TnI in the troponin complex in solution.
Biophysical Journal, 90 (7).
pp. 2436-2444.
ISSN 0006-3495
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Abstract
The determination of crystal structures of the troponin complex (Takeda et al. 2003. Nature. 424:35–41; Vinogradova et al. 2005. Proc. Natl. Acad. Sci. USA. 102:5038–5043) has advanced knowledge of the regulation of muscle contraction at the molecular level. However, there are domains important for actin binding that are not visualized. We present evidence that the C-terminal region of troponin I (TnI residues 135–182) is flexible in solution and has no stable secondary structure. We use NMR spectroscopy to observe the backbone dynamics of skeletal [2H, 13C, 15N]-TnI in the troponin complex in the presence of Ca2+ or EGTA/Mg2+. Residues in this region give stronger signals than the remainder of TnI, and chemical shift index values indicate little secondary structure, suggesting a very flexible region. This is confirmed by NMR relaxation measurements. Unlike TnC and other regions of TnI in the complex, the C-terminal region of TnI is not affected by Ca2+ binding. Relaxation measurements and reduced spectral density analysis are consistent with the C-terminal region of TnI being a tethered domain connected to the rest of the troponin complex by a flexible linker, residues 137–146, followed by a collapsed region with at most nascent secondary structure.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) |
| Depositing User: | Rachel Smith |
| Date Deposited: | 15 Feb 2011 12:27 |
| Last Modified: | 24 Sep 2024 09:45 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/21376 |
| DOI: | 10.1529/biophysj.105.076216 |
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