pH dependence of binding benzohquinoline and humic acid and effects on fluorescence quenching

Hsieh, Ping-Chieh, Hsu, Shih-Han, Lee, Chon-Lin and Brimblecombe, Peter (2010) pH dependence of binding benzohquinoline and humic acid and effects on fluorescence quenching. Environmental Toxicology and Chemistry, 29 (8). pp. 1696-1702. ISSN 1552-8618

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Abstract

The binding constant (KDOC) between humic acid and the nitrogen-containing polycyclic aromatic compound (N-PAC), benzo[h]quinoline, was measured at varying pH levels using fluorescence quenching (FQ). Because fluorescence characteristics of benzo[h]quinoline change with pH, determination required two optimum sets of excitation and emission wavelength pairs. A simple mixing model was used to eliminate the inherent fluorescence interference between benzo[h]quinoline and its protonated form, benzo[h]quinolinium, when estimating binding constants. Hydrophobic interaction is likely to control the binding between humic acid and benzo[h]quinoline and benzo[h]quinolinium, in lower and higher pH ranges (pH

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Environmental Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Groups > Marine and Atmospheric Sciences (former - to 2017)
Depositing User:	Rosie Cullington
Date Deposited:	03 Feb 2011 15:46
Last Modified:	29 May 2024 15:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/20313
DOI:	10.1002/etc.220

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