Characterisation of fractalkine/CX3CL1 and fractalkine receptor (CX3CR1) expression in abdominal aortic aneurysm disease

Patel, A., Jagadesham, V., Porter, K. E., Scott, D. and Carding, S. (2008) Characterisation of fractalkine/CX3CL1 and fractalkine receptor (CX3CR1) expression in abdominal aortic aneurysm disease. European Journal of Vascular and Endovascular Surgery, 36 (1). pp. 20-27. ISSN 1532-2165

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Abstract

Objectives: Fractalkine (CX3CL1) promotes adhesion and extravasation of leucocytes through interactions with fractalkine receptor (CX3CR1) expressed on CD56+/CD16+ NK cells and CD8+ T cells. The current study aims to test the hypothesis the CX3CL1–CX3CR1 interaction contributes to the inflammatory infiltrate in AAA tissue. Design and methods: Immunohistochemistry (IHC) was used to define expression of CX3CR1 in AAA tissue. Multi-parametric flow cytometry (FC) was used to determine CX3CR1 expression on T-cells (CD3+) and NK cells (CD56+) from AAA tissue and peripheral blood of AAA patients and healthy controls. Regulation of CX3CL1 expression by vascular endothelial (vEC) and smooth muscle cells (vSMC) was examined in vitro using primary cell cultures. Results: CX3CR1+ cells were detected in 19/28 AAA tissue samples and predominately localised in the adventitia. PBMCs from patients with AAA demonstrated higher percentages of CX3CR1+ NK cells (60.0–88.6%) and T cells (7.5–39.4%) compared with healthy controls. Furthermore, the frequency of CX3CR1+ NK cells (91%) and T cells (94%) in inflammatory AAA tissue were higher than in atherosclerotic AAA tissue. The pro-inflammatory cytokine TNFα increased expression of fractalkine by vSMC and vEC. Conclusion: CX3CL1+ and CX3CR1+ cells are present in AAA disease and their interaction may contribute to the recruitment of inflammatory cells seen in AAA tissue.

Item Type:	Article
Additional Information:	Acknowledgements: This work was supported by grants from the School of Medicine, University of Leeds and by a British Heart Foundation Clinical Research Training Fellowship (VPJ).
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	EPrints Services
Date Deposited:	25 Nov 2010 11:13
Last Modified:	24 Sep 2024 09:34
URI:	https://ueaeprints.uea.ac.uk/id/eprint/15084
DOI:	10.1016/j.ejvs.2008.01.014

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