Identification of SNAREs that mediate zymogen granule exocytosis

Pickett, James A., Campos-Toimil, Manuel, Thomas, Paul and Edwardson, J. Michael (2007) Identification of SNAREs that mediate zymogen granule exocytosis. Biochemical and Biophysical Research Communications, 359 (3). pp. 599-603. ISSN 1090-2104

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Abstract

A secretagogue-stimulated pancreatic acinar cell releases digestive enzymes from its apical pole. We attempted to identify the SNAREs involved in zymogen granule exocytosis. Antibodies against syntaxins 2 and 3, SNAP-23 and VAMP 8, and the corresponding recombinant SNAREs, inhibited amylase secretion from streptolysin O-permeabilised acini; other anti-SNARE antibodies and SNAREs had no effect. Botulinum neurotoxin C, which cleaved syntaxin 2 and (to a lesser extent) syntaxin 3, but not syntaxins 4, 7 or 8, also inhibited exocytosis. We propose that syntaxin 2, SNAP-23 and VAMP 8 mediate primary granule–plasma membrane fusion. Syntaxin 3 may be involved in secondary granule–granule fusion.

Item Type:	Article
Faculty \ School:	Faculty of Science
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:38
Last Modified:	24 Sep 2024 09:43
URI:	https://ueaeprints.uea.ac.uk/id/eprint/1431
DOI:	10.1016/j.bbrc.2007.05.128

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