Sexton, D. W., Al-Rabia, M., Blaylock, M. G. and Walsh, G. M. (2004) Phagocytosis of apoptotic eosinophils but not neutrophils by bronchial epithelial cells. Clinical and Experimental Allergy, 34 (10). pp. 1514-1524. ISSN 1365-2222
Full text not available from this repository.Abstract
Background: We have previously demonstrated that human bronchial epithelial cells engulf apoptotic eosinophils. Objectives: To compare and contrast the phagocytic capabilities of monocyte-derived macrophage and primary airway epithelial cells for apoptotic granulocytes. Results: Here we compared phagocytosis of human apoptotic eosinophils and neutrophils by small and large airway epithelial cells (SAEC and LAEC) and monocyte-derived macrophages. Confocal microscopy of F-actin staining and scanning and transmission electron microscopy revealed phagocytic cup formation around apoptotic eosinophils by airway epithelial cells (AEC) membranes with evidence of their digestion. Resting and cytokine-stimulated AEC did not recognize and ingest apoptotic neutrophils. The latter were phagocytosed by macrophages that exhibited greater ingestion of and higher capacity for, apoptotic eosinophils over apoptotic neutrophils. Cytochalasin D completely abolished uptake of apoptotic eosinophils by SAEC, LAEC or macrophage monolayers. Ligation of epithelial cell CD44 receptors for 24 h increased phagocytosis of apoptotic eosinophils by SAEC and LAEC with a potency comparable with that of IL-1. Phagocytosis was a specific receptor-mediated process involving integrin- (αvβ3, αvβ5, CD36), phosphatidylserine receptor- and lectin-dependent mechanisms. No significant differences were observed in avarice for apoptotic eosinophils by SAEC or LAEC either resting, CD44 monoclonal antibodies- or cytokine- stimulated, or in their usage and expression of recognition receptors. Conclusion: These findings further suggest and define an important role for the bronchial epithelium in the selective removal of apoptotic eosinophils from the airways in asthma.
Item Type: | Article |
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Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Norwich Clinical Trials Unit |
Depositing User: | EPrints Services |
Date Deposited: | 25 Nov 2010 11:11 |
Last Modified: | 31 Jul 2024 17:23 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/13643 |
DOI: | 10.1111/j.1365-2222.2004.02054.x |
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