The Npro product of classical swine fever virus interacts with IkappaBalpha, the NF-kappaB inhibitor

Doceul, Virginie, Charleston, Bryan, Crooke, Helen, Reid, Elizabeth, Powell, Penny ORCID: https://orcid.org/0000-0002-5347-0490 and Seago, Julian (2008) The Npro product of classical swine fever virus interacts with IkappaBalpha, the NF-kappaB inhibitor. Journal of General Virology, 89 (8). pp. 1881-1889. ISSN 1465-2099

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Abstract

Classical swine fever virus (CSFV) belongs to the genus Pestivirus and is the causative agent of classical swine fever, a haemorrhagic disease of pigs. The virus replicates in host cells without activating interferon (IFN) production and has been reported to be an antagonist of double-stranded RNA-induced apoptosis. The N-terminal protease (N(pro)) of CSFV is responsible for this evasion of the host innate immune response. In order to identify cellular proteins that interact with the N(pro) product of CSFV, a yeast two-hybrid screen of a human library was carried out, which identified IkappaBalpha, the inhibitor of NF-kappaB, a transcription factor involved in the control of apoptosis, the immune response and IFN production. The N(pro)-IkappaBalpha interaction was confirmed using yeast two-hybrid analysis and additional co-precipitation assays. It was also shown that N(pro) localizes to both the cytoplasmic and nuclear compartments in stably transfected cells and in CSFV-infected cells. Following stimulation by tumour necrosis factor alpha, PK-15 cell lines expressing N(pro) exhibited transient nuclear accumulation of pIkappaBalpha, but no effect of CSFV infection on IkappaBalpha localization or NF-kappaB p65 activation was observed.

Item Type:	Article
Uncontrolled Keywords:	nf-kappa b,animals,viral proteins,humans,two-hybrid system techniques,i-kappa b proteins,kidney,endopeptidases,cell line,classical swine fever virus,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	EPrints Services
Date Deposited:	25 Nov 2010 11:09
Last Modified:	19 Oct 2023 00:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/12518
DOI:	10.1099/vir.0.83643-0

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