Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis

Korycka-Machala, Malgorzata, Brzostek, Anna, Rozalska, Sylwia, Rumijowska-Galewicz, Anna, Dziedzic, Renata, Bowater, Richard ORCID: https://orcid.org/0000-0002-2745-7807 and Dziadek, Jaroslaw (2006) Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis. FEMS Microbiology Letters, 258 (1). pp. 83-91. ISSN 1574-6968

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Abstract

Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and in their response to chemicals that damage DNA. The results provide in vivo evidence that only NHEJ is required for the repair of noncompatible DNA ends. By contrast, the response of mycobacteria to mitomycin C preferentially involved a RecA-dependent pathway.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:37
Last Modified:	13 Nov 2024 00:35
URI:	https://ueaeprints.uea.ac.uk/id/eprint/1146
DOI:	10.1111/j.1574-6968.2006.00199.x

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