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ToolsLi, Leyuan and Figeys, Daniel (2020) Proteomics and Metaproteomics Add Functional, Taxonomic and Biomass Dimensions to Modeling the Ecosystem at the Mucosal-luminal Interface. Molecular and Cellular Proteomics, 19 (9). pp. 1409-1417. ISSN 1535-9476
Full text not available from this repository. (Request a copy)Abstract
Recent efforts in gut microbiome studies have highlighted the importance of explicitly describing the ecological processes beyond correlative analysis. However, we are still at the early stage of understanding the organizational principles of the gut ecosystem, partially because of the limited information provided by currently used analytical tools in ecological modeling practices. Proteomics and metaproteomics can provide a number of insights for ecological studies, including biomass, matter and energy flow, and functional diversity. In this Mini Review, we discuss proteomics and metaproteomics-based experimental strategies that can contribute to studying the ecology, in particular at the mucosal-luminal interface (MLI) where the direct host-microbiome interaction happens. These strategies include isolation protocols for different MLI components, enrichment methods to obtain designated array of proteins, probing for specific pathways, and isotopic labeling for tracking nutrient flow. Integration of these technologies can generate spatiotemporal and site-specific biological information that supports mathematical modeling of the ecosystem at the MLI.
| Item Type: | Article |
|---|---|
| Additional Information: | Publisher Copyright: © 2020 Li and Figeys. |
| Uncontrolled Keywords: | analytical chemistry,biochemistry,molecular biology ,/dk/atira/pure/subjectarea/asjc/1600/1602 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 27 Mar 2026 10:30 |
| Last Modified: | 27 Mar 2026 10:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/102605 |
| DOI: | 10.1074/mcp.R120.002051 |
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