Associations between Cellular Energy and Pediatric Inflammatory Bowel Disease Patient Response to Treatment

Starr, Amanda E., Deeke, Shelley A., Ning, Zhibin, De Nanassy, Joseph, Singleton, Ruth, Benchimol, Eric I., Mack, David R., Stintzi, Alain and Figeys, Daniel (2021) Associations between Cellular Energy and Pediatric Inflammatory Bowel Disease Patient Response to Treatment. Journal of Proteome Research, 20 (9). pp. 4393-4404. ISSN 1535-3893

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Abstract

Inflammatory bowel diseases (IBDs), including Crohn's disease (CD) and ulcerative colitis, are chronic diseases of the gastrointestinal tract, with an unknown etiology, that affect over 6.8 million people worldwide. To characterize disease pathogenesis, proteomic and bioinformatic analyses were performed on colon biopsies collected during diagnostic endoscopy from 119 treatment-naïve pediatric patients, including from 78 IBD patients and 41 non-IBD patients who served as controls. Due to the presence of noninflamed and/or inflamed regions in IBD patients, up to two biopsies were obtained from IBD patients as compared to a single noninflamed biopsy from non-IBD pediatric control patients. Additional biopsies were obtained and analyzed from 33 of the IBD patients after IBD-directed therapeutic intervention for comparison of pre- and post-treatment proteomes. SuperSILAC was utilized to perform quantitative analysis of homogenized tissues, which were processed by filter-aided sample preparation. Hierarchical clustering and principal component analyses revealed proteomic patterns that distinguished inflamed from noninflamed tissues independent of therapy. Gene ontology revealed that proteins downregulated in inflammation are associated with metabolism, whereas upregulated proteins contribute to protein processing. A comparison of pre- and post-treatment proteomes from CD patients identified over 100 proteins that are significantly different between patients who responded and those who did not respond to therapy, including creatine kinase B and basigin.

Item Type:	Article
Additional Information:	Publisher Copyright: © 2021 American Chemical Society.
Uncontrolled Keywords:	biomarkers,inflammatory bowel diseases (ibds),pediatrics,chemistry(all),biochemistry ,/dk/atira/pure/subjectarea/asjc/1600
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	26 Mar 2026 14:30
Last Modified:	26 Mar 2026 14:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102600
DOI:	10.1021/acs.jproteome.1c00341

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