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ToolsCheng, Kai, Ning, Zhibin, Zhang, Xu, Li, Leyuan, Liao, Bo, Mayne, Janice and Figeys, Daniel (2020) MetaLab 2.0 Enables Accurate Post-Translational Modifications Profiling in Metaproteomics. Journal of the American Society for Mass Spectrometry, 31 (7). pp. 1473-1482. ISSN 1044-0305
Full text not available from this repository. (Request a copy)Abstract
Studying the structure and function of microbiomes is an emerging research field. Metaproteomic approaches focusing on the characterization of expressed proteins and post-translational modifications (PTMs) provide a deeper understanding of microbial communities. Previous research has highlighted the value of examining microbiome-wide protein expression in studying the roles of the microbiome in human diseases. Nevertheless, the regulation of protein functions in complex microbiomes remains underexplored. This is mainly due to the lack of efficient bioinformatics tools to identify and quantify PTMs in the microbiome. We have developed comprehensive software termed MetaLab for the data analysis of metaproteomic data sets. Here, we build an open search workflow within MetaLab for unbiased identification and quantification of unmodified peptides as well as peptides with various PTMs from microbiome samples. This bioinformatics platform provides information about proteins, PTMs, taxa, functions, and pathways of microbial communities. The performance of the workflow was evaluated using conventional proteomics, metaproteomics from mouse and human gut microbiomes, and modification-specific enriched data sets. Superior accuracy and sensitivity were obtained simultaneously by using our method compared with the traditional closed search strategy.
| Item Type: | Article |
|---|---|
| Additional Information: | Publisher Copyright: © 2020 Springer New York LLC. All rights reserved. |
| Uncontrolled Keywords: | bioinformatics,metaproteomics,open search,post-translational modification,structural biology,spectroscopy,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1300/1315 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 26 Mar 2026 11:30 |
| Last Modified: | 26 Mar 2026 11:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/102592 |
| DOI: | 10.1021/jasms.0c00083 |
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