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ToolsLi, Leyuan, Zhang, Xu, Ning, Zhibin, Mayne, Janice, Moore, Jasmine I., Butcher, James, Chiang, Cheng Kang, Mack, David, Stintzi, Alain and Figeys, Daniel (2018) Evaluating in Vitro Culture Medium of Gut Microbiome with Orthogonal Experimental Design and a Metaproteomics Approach. Journal of Proteome Research, 17 (1). pp. 154-163. ISSN 1535-3893
Full text not available from this repository. (Request a copy)Abstract
In vitro culture based approaches are time- and cost-effective solutions for rapidly evaluating the effects of drugs or natural compounds against microbiomes. The nutritional composition of the culture medium is an important determinant for effectively maintaining the gut microbiome in vitro. This study combines orthogonal experimental design and a metaproteomics approach to obtaining functional insights into the effects of different medium components on the microbiome. Our results show that the metaproteomic profile respond differently to medium components, including inorganic salts, bile salts, mucin, and short-chain fatty acids. Multifactor analysis of variance further revealed significant main and interaction effects of inorganic salts, bile salts, and mucin on the different functional groups of gut microbial proteins. While a broad regulating effect was observed on basic metabolic pathways, different medium components also showed significant modulations on cell wall, membrane, and envelope biogenesis and cell motility related functions. In particular, flagellar assembly related proteins were significantly responsive to the presence of mucin. This study provides information on the functional influences of medium components on the in vitro growth of microbiome communities and gives insight on the key components that must be considered when selecting and optimizing media for culturing ex vivo microbiotas.
| Item Type: | Article |
|---|---|
| Additional Information: | The Supporting Information is available free of charge on the ACS Publications website at DOI: 10.1021/acs.jproteo-me.7b00461. D.F. acknowledges a Canada Research Chair in Proteomics and Systems Biology. |
| Uncontrolled Keywords: | functional insights,gut microbiome,in vitro culturing,metaproteomics,orthogonal design,chemistry(all),biochemistry ,/dk/atira/pure/subjectarea/asjc/1600 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 23 Mar 2026 10:30 |
| Last Modified: | 29 Mar 2026 06:35 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/102528 |
| DOI: | 10.1021/acs.jproteome.7b00461 |
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