Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation

Li, Henghui, Li, Leyuan, Cheng, Kai, Ning, Zhibin, Mayne, Janice, Zhang, Xu, Walker, Krystal, Chen, Rui, Twine, Susan, Li, Jianjun and Figeys, Daniel (2019) Chemoenzymatic Method for Glycoproteomic N-Glycan Type Quantitation. Analytical Chemistry, 92 (1). pp. 1618-1627. ISSN 0003-2700

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Abstract

Glycosylation is one of the most important post-translational modifications in biological systems. Current glycoproteome methods mainly focus on qualitative identification of glycosylation sites or intact glycopeptides. However, the systematic quantitation of glycoproteins has remained largely unexplored. Here, we developed a chemoenzymatic method to quantitatively investigate N-glycoproteome based on the N-glycan types. Taking advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-glycan types of structures linked on each glycopeptide, including high-mannose/hybrid, biantennary, and triantennary with/without core fucose, were quantified. As a proof of principle, the glycoproteomic N-glycan type quantitative (glyco-TQ) method was first used to determine the N-glycan type composition of the immunoglobulin G1 (IgG1) Fc fragment. Then we applied the method to analyze the glycan type profile of proteins from the breast cancer cell line MCF7, and we quantitatively revealed the N-glycan type microheterogeneity at the glycopeptide and glycoprotein level. The novel quantitative strategy to evaluate the relative intensity of the six states of N-glycan type glycosylation on each site provides a new avenue to investigate the function of glycoproteins in broad areas, such as cancer biomarker research, pharmaceuticals characterization, and antiglycan vaccine development.

Item Type:	Article
Additional Information:	Publisher Copyright: © 2019 American Chemical Society.
Uncontrolled Keywords:	analytical chemistry ,/dk/atira/pure/subjectarea/asjc/1600/1602
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	20 Mar 2026 15:30
Last Modified:	20 Mar 2026 15:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/102516
DOI:	10.1021/acs.analchem.9b04937

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